FISH images for the unbalanced genes and centromeric targets in the megaesophagus specimens. Dual-target, dual-color color hybridization in chagasic megaesophagus. A. Loss of CDKN2A (red signal) and centromere 9 (green signal); B. Loss of EGFR (red signal) and centromere 7 (green signal); C. Loss of FHIT (red signal) and centromere 3 (green signal); D. Gain of TP63 (red signal) and loss of PIK3CA (green signal). Arrows indicated cells with abnormal copy numbers of the genes.