Figure 2From: Differential detection of nuclear envelope autoantibodies in primary biliary cirrhosis using routine and alternative methods Validation of substrates used in IIF for the detection of ANEA. Hep2 slides, HeLa and HepG2 cells fixed with formaldehyde either 4% (upper panel) or 1% (lower panel) and purified nuclei from HepG2 cells and rat liver were incubated with various PBC sera (s54, s32, s16, s189 and s201) and secondary anti-human FITC-labeled IgG antibodies, as described in Materials and Methods.Back to article page