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Table 1 Characteristic values relating to the expression of each gene considered by Real time PCR

From: Bax Inhibitor-1 down-regulation in the progression of chronic liver diseases

Gene

Threshold

Slope

Efficiency

E = (10 -1/slope )-1

R 2

Range

(copies/μl)

β-actin (n.24)

0.078

-3.34 ± 0.03

98.37 ± 3.43

0.998

10 8 -10 2

Bax (n.15)

0.104

-3.51 ± 0.05

94.67 ± 1.99

0.998

10 8 -10 3

BI-1 (n.18)

0.144

-3.48 ± 0.03

93.48 ± 1.34

0.999

10 8 -10 2

  1. Threshold value, manually adjusted, was maintained for all the standard curve analyzed. Table report the mean ±SD of the slope and efficiency value relative to n. 24, 15 and 18 standard curves of β-actin, Bax and BI-1 respectivelly. Efficiency was calculated from the slope using the equation E = (10-1/slope) -1. The correlation coefficient (square values R2) and range of standard curves are also reported.