Table 1 Tumorigenicity experiments of PLC/PRF/5 sphere-forming cells and parental cells in NOD/SCID mice
From: Sphere-forming cell subpopulations with cancer stem cell properties in human hepatoma cell lines
Cell type | Cell numbers injected | Tumor incidence†| Latency(days)‡ |
|---|---|---|---|
Sphere-forming cells of the 3rd generation | 2 × 102 | 0/3 | - |
|  | 5 × 102 | 1/3 | 35 |
|  | 1 × 103 | 3/3 | 23 |
|  | 2.5 × 103 | 3/3 | 23 |
|  | 5 × 103 | 3/3 | 20 |
|  | 1 × 104 | 3/3 | 16 |
|  | 5 × 104 | 2/2 | 9 |
|  | 1 × 105 | 2/2 | 7 |
|  | 2 × 105 | 2/2 | 7 |
Sphere-forming cells of the 6th generation | 2 × 102 | 0/3 | - |
|  | 5 × 102 | 2/3 | 49 |
|  | 1 × 103 | 3/3 | 28 |
Sphere-forming cells of the 9th generation | 2 × 102 | 0/3 | - |
|  | 5 × 102 | 1/3 | 40 |
|  | 1 × 103 | 3/3 | 30 |
PLC/PRF/5 parental cells | 5 × 104 | 0/3 | - |
|  | 1 × 105 | 0/3 | - |
|  | 2 × 105 | 2/3 | 30 |
|  | 5 × 105 | 3/3 | 16 |
|  | 1 × 106 | 2/2 | 9 |