Gene | Primer sequence | Annealing | Restriction enzyme |
---|---|---|---|
MTHFR C677T | F: 5'-TGA AGG AGA AGG TGT CTG CGG GA-3' R: 5'-AGG ACG GTG CGG TGA GAG TG-3' | 62°C | Hinf I |
CYP2E1 G1259C | F: 5'-CCA GTC GAG TCT ACA TTG TCA-3' R: 5'-TTC ATT CTG TCT TCT AAC TGG-3' | 55°C | PstI |
hOGG1 C326G | F: 5'-GGA AGG TGC TTG GGG AAT-3' R: 5'-ACT GTC ACT AGT CTC ACC AG-3' | 58°C | Fnu4HI |
MPO G463A | F: 5'-CGG TAT AGG CAC ACA ATG GTG AG-3' R: 5'-GCA ATG GTT CAA GCG ATT C-3' | 62°C | AciI |
ALDH2 | F1: 5'-TCA TGC CAT GGC AAC TCC AGC-3' R1: 5'-CCC ACA CTC ACA GTT TTC TCT TC-3' F2: 5'-TAC GGG CTG CAG GCA TAC ACT A-3 R2: 5'-TGA TCC CCA GCA GGT CCT GAA-3' | 60°C |  |