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Figure 3 | BMC Gastroenterology

Figure 3

From: Longitudinal fluctuations in PD1 and PD-L1 expression in association with changes in anti-viral immune response in chronic hepatitis B

Figure 3

Correlation between PD1, PD-L1 expression and serum pentamer frequency, ALT level, HBsAg and HBV-DNA load. (A) Percentage of core, even and pol from T1 to T4. The frequency of pentamers including core 18–27, even 335–343 and pol 575–583 increased upon initial of inflammation (T1), reached peak level during climax of inflammation (T2) and decreased gradually during regression of inflammation (T4). (core, even and pol, n = 15, P T1 vs T2 <0.01, P T2 vs T3 <0.05, P T3 vs T4 < 0.01) (B) Fluctuation of sALT, HBsAg and HBV-DNA from T1 to T4. From T1 to T2, serum ALT level increased significantly, in parallel with pentamer cells clonal expansion, and decreased significantly in concordance with pentamer cells clonal contraction from T3 to T4. ( sALT, n = 15, P T1 vs T2 <0.01, P T2 vs T3 <0.05, P T3 vs T4 <0.05) Serum HBsAg and HBV-DNA load decreased during inflammation flare up period. (HBsAg and HBV-DNA copies, n = 15, P T1 vs T2 <0.01, P T2 vs T3 <0.01, P T3 vs T4 < 0.01) (C) Percentage of circulating PD1 and PD-L1 from T1 to T4. From T1 to T2 period, in parallel with pentamer cells clonal expansion, circulating PD1 and PD-L1 expression increased significantly. From T3 to T4 period, parallel with pentamer cells clonal contraction, circulating PD1 and PD-L1 expression decreased significantly. (P T1 vs T2 <0.01, P T2 vs T3 <0.05, P T3 vs T4 < 0.01 n = 5) (D) The number of intra hepatic CD8, PD1 and PD-L1 positive cells from T2 to T4. DuringT2 period, the number of intra-hepatic PD1, PD-L1 and CD8 reached the highest level and they were decreased significantly in T3 period. From T3 to T4 period, number of intra-hepatic PD-1 and CD8 positive cells further decreased significantly, however PD-L1 did not. (PD1,PD-L1 and CD8, PT2 vs T3 < 0.01; PD1 and CD8, P T3 vs T4 < 0.01; PD-L1, P T3 vs T4 > 0.05; n = 5).

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