Figure 2

Analysis of chronic pancreatitis using the approaches of histopathological, immunohistochemical and histochemical assay. Histopathology in the pancreatic tissue of mice: (A) Pancreas in control mice showing morphologically normal pancreatic parenchyma (acini and islets as well interlobular ducts). (B) Extensive chronic pancreatitis in caerulein induced chronic pancreatitis mice showing loss of acini and stromal fibrosis. (C) Mild chronic pancreatitis in mice with sulindac treatment. Amylase and CK19 expression in the pancreas: (D) Pancreas from control mice showing mainly composed of acinar cells and scattered islets, CK19 only positive in the ducts. (E) In caerulein induced chronic pancreatitis mice, the amylase positive acinar cells were instead by CK19 positive ductal cells. (F) Chronic pancreatitis mice treated with sulindac exhibiting increased amylase positive area and decreased CK19 positive area. Trichrome stain highlighting stromal fibrosis in the pancreas: (G) Trichrome stained fibroconnective tissue (blue color) only in the interlobular areas of a normal pancreas in control mice. (H) Caerulein induced chronic pancreatitis mice showing extensive fibrosis in pancreatic parenchyma. (I) Chronic pancreatitis mice with sulindac treatment exhibit much less stromal fibrosis in the pancreas. MPO-labeled neutrophils in the pancreas: (J) Pancreas from control mice showed rare MPO-labeled neutrophils. (K) Caerulein induced chronic pancreatitis mice displayed intense MPO-labeled neutrophils in the areas of pancreatitis. (L) Chronic pancreatitis mice with sulindac treatment exhibited decreased MPO-positive neutrophils. Mac-3-labeled macrophages in the pancreas: (M) Pancreas from control mice showed no Mac-3-labeled inflammatory cells. (N) Caerulein induced chronic pancreatitis mice displayed intense Mac-3-labeled macrophage in the areas of pancreatitis. (O) Chronic pancreatitis mice with sulindac treatment exhibited decreased Mac-3-positive macrophages.