Figure 4
Effect of 1,25(OH) 2 D 3 on colonic epithelial junctions. (A) Expressions of zo-1, occludin and claudin-1 were examined by immunostaining for control, model, and 1,25(OH)2D3-treated group. Magnification: ×200. (B) Immunoflorescent stain with anti-claudin-1 (red), anti-oocludin (red) and anti-zo-1 (green) antibody was shown. Notes of the zo-1, occludin and claudin-1 staining were markedly decreased in the model group compared with that of the control and 1,25(OH)2D3-treated group. (C) Expressions of zo-1, occludin and clandin-1 genes were performed and shown by real time Q-PCR analysis. (D and E) Expressions of TJ proteins in the colon were assessed by Western blot. In model group, expressions of zo-1, occludin and claudin-1 were decreased markedly compared with that of control group. Data were expressed as mean ± SD. a P < 0.01 vs model group; b P < 0.01 vs control group.