Lactobacillus acidophilus CRL 1014 improved “gut health” in the SHIME®reactor

Sivieri, Katia; Morales, Martha L Villarreal; Adorno, Maria A Tallarico; Sakamoto, Isabel Kimiko; Saad, Susana M Isay; Rossi, Elizeu A

doi:10.1186/1471-230X-13-100

BMC Gastroenterology

Table 2 Average plate count measurements (±SEM), expressed in log CFU mL ¹ , for the different microbial groups, SHIME compartments and periods

From: Lactobacillus acidophilus CRL 1014 improved “gut health” in the SHIME^®reactor

	Basal		Treatment			Washout
	^1stweek	^2ndweek	^1stweek	^2ndweek	^3thweek	^4thweek	^1stweek	^2ndweek
Ascending colon
Enterococcus spp	5.20^a ± 0.01	5.40^a ± 0.02	6.30^b ± 0.10	7.25^c ± 0.04	7.20^c ± 0.06	8.05^d ± 0.21	7.00^c ± 0.02	7.00^c ±0.01
enterobacteria	7.35^d ± 0.41	6.00^c ± 0.30	6.20^c ± 0.32	6.40^c ± 0.01	6.72^c ± 0.22	6.80^c ± 0.32	5.68^b ± 0.01	4.20^a ± 0.05
Lactobacillus spp	5.17^a ± 0.30	5.23^a ± 0.22	7.78^b ± 0.03	7.77^b ± 0.01	8.28^c ± 0.05	7.88^bc ± 0.01	7.20^b ± 0.04	5.40^a ± 0.04
Bifidobacterium spp	5.80^a ± 0.22	6.90^b ± 0.55	7.77^c ± 0.01	7.86^c ± 0.05	7.88^c ± 0.06	7.97^c ± 0.06	7.68^c ± 0.03	7.61^c ± 0.04
Clostridium spp	7.68^a ± 0.02	8.32^b ± 0.02	8.69^b ± 0.01	9.39^bc ± 0.01	12.39^d ± 0.01	12.30^d ± 0.02	12.40^d ± 0.01	12.39^d ± 0.01
Total aerobes	7.14^a ± 0.41	7.84^a ± 0.05	7.72^a ± 0.01	7.42^a ± 0.02	7.55^a ± 0.04	7.32^a ± 0.01	7.30^a ± 0.01	7.42^a ± 0.05
Facultative anaerobes	7.66^a ± 0.03	8.20^a ± 0.02	7.80^a ± 0.02	7.60^a ± 0.04	7.40^a ± 0.01	7.30^a ± 0.03	7.46^a ± 0.02	7.50^a ± 0.01
Transverse colon
Enterococcus spp	4.14^a ± 0.22	4.44^a ± 0.02	7.19^b ± 0.01	6.84^b ± 0.01	7.45^b ± 0.03	7.50^b ± 0.05	7.00^b ± 0.04	7.00^b ± 0.01
enterobacteria	6.23^b ± 0.46	5.15^a ± 0.15	5.00^a ± 0.01	6.30^b ± 0.01	6.00^b ± 0.01	6.00^b ± 0.01	6.84^b ± 0.01	6.86^b ± 0.02
Lactobacillus spp	5.20^a ± 0.02	5.19^a ± 0.02	7.72^b ± 0.02	7.35^b ± 0.05	7.24^b ± 0.01	7.17^b ± 0.05	8.33^b ± 0.05	8.40^b ± 0.01
Bifidobacterium spp	5.80^a ± 0.07	5.00^a ± 0.01	7.18^b ± 0.14	7.86^b ± 0.05	7.43^b ± 0.01	7.31^b ± 0.14	7.79^b ± 0.05	7.80^b ± 0.02
Clostridium spp	6.61^a ± 0.01	6.62^a ± 0.01	7.27^a ± 0.01	8.39^b ± 0.01	8.26^b ± 0.03	8.53^b ± 0.23	10.39^c ± 0.01	10.40^c ± 0.01
Total aerobes	7.00^a ± 0.02	7.20^a ± 0.20	7.74^a ± 0.02	7.32^a ± 0.11	7.82^a ± 0.02	7.86^a ± 0.03	8.69^b ± 0.22	8.64^b ± 0.10
Facultative anaerobes	6.32^a ± 0.07	6.30^a ± 0.01	6.00^a ± 0.41	6.20^a ± 0.13	6.10^a ± 0.22	6.30^a ± 0.01	7.80^ab ± 0.01	8.10^b ± 0.11
Descending colon
Enterococcus spp	4.30^a ± 0.02	4.32^a ± 0.01	7.08^b ± 0.01	6.66^b ± 0.02	6.75^b ± 0.05	6.76^b ± 0.04	7.08^b ± 0.02	7.06^b ± 0.01
enterobacteria.	7.46^b ± 0.06	6.00^a ± 0.01	6.15^a ± 0.15	6.30^a ± 0.01	6.00^a ± 0.01	6.00^a ± 0.01	5.67^ab ± 0.01	5.70^ab ± 0.01
Lactobacillus spp	5.36^a ± 0.01	5.38^a ± 0.02	7.46^b ± 0.02	7.23^b ± 0.06	7.13^b ± 0.09	6.53^b ± 0.53	7.20^b ± 0.01	7.27^b ± 0.08
Bifidobacterium spp	6.79^b ± 0.04	5.72^a ± 0.12	7.15^c ± 0.01	7.53^c ± 0.01	6.84^bc ± 0.15	7.37^c ± 0.03	7.55^c ± 0.01	7.47^c ± 0.01
Clostridium spp	7.72^a ± 0.01	7.74^a ± 0.06	8.48^b ± 0.01	9.39^c ± 0.01	10.50^d ± 0.15	10.50^d ± 0.02	10.34^d ±0.02	10.38^d ± 0.44
Total aerobes	7.24^a ± 0.07	7.53^a ± 0.01	7.44^a ± 0.01	7.47^a ± 0.42	7.58^a ± 0.09	7.86^a ± 0.05	8.00^a ± 0.01	8.20^a ± 0.45
Facultative anaerobes	7.39^b ± 0.11	6.30^a ± 0.01	6.59^a ± 0.01	6.39^a ± 0.02	6.40^a ± 0.01	6.00^a ± 0.03	7.69^b ± 0.01	7.63^b ± 0.05

Different letters indicate significantly different results (p < 0.01) in same microbial group and same compartment (ascending, transverse, and descending colon), during basal, treatment and washout period.

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