Table 1 Real time RT-PCR primers
From: Guanylate cyclase C limits systemic dissemination of a murine enteric pathogen
Primers | Sequences |
|---|---|
Gn | For 5’- CAC TGT GCA GGA TGG AGA C-3’ |
Rev 5’-CTC GGC GTT GGG TTT CT-3’ | |
CFTR | For 5’-GCA CAG CAG CTC AAA CAA CTG GAA-3’ |
Rev 5’-TTC TCA TTT GGA ACC AGC GCA AGC-3’ | |
IL-22 | For 5’-AAA CTG TTC CGA GGA GTC AGT GCT |
Rev 5’-GCT GAG CTG ATT GCT GAG TTT GGT-3’ | |
CXCL9 | For 5’-TTT CAT CAC GCC CTT GAG CCT AGT-3’ |
Rev 5’-TTT GGT GAC GTG AGC CTC AGA AGT-3’ | |
GAPDH | For 5’-ACC ACA GTC CAT GCC ATC AC-3’ |
Rev 5’-TCC ACC ACC CTG TTG CTG TA-3’ |