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Figure 1 | BMC Gastroenterology

Figure 1

From: TNF-α and LPA promote synergistic expression of COX-2 in human colonic myofibroblasts: role of LPA-mediated transactivation of upregulated EGFR

Figure 1

TNF-α Potentiates LPA-mediated EGFR Phosphorylation at Y1068. Panel A: Confluent 18Co cells were exposed to 10 μM LPA for various times (broken lines), with or without pre-incubation with 10 ng/ml TNF-α for 18 h (indicated by solid line). Western blot analysis was used with antibodies that detect EGFR and EGFR phosphorylation at Y1068. Densitometry analysis shows the mean ± S.E. (n ≥ 3), expressed as percentage of the maximal level of EGFR Y1068 phosphorylation. In all experiments, equal protein loading was verified using α-SMA antibody. Panel B: 18Co cells were incubated with EGF (5 ng/ml) for 5 or 60 min (broken lines), with or without pre-incubation with 10 ng/ml TNF-α for 18 h (indicated by solid line). Western blot analysis used an antibody detecting EGFR phosphorylation at Y1068. The results are representative of three separate experiments. Panel C: 18Co cells were exposed to 10 μM LPA for 8 h (broken lines), without or with pre-incubation with 10 ng/ml TNF-α for 18 h (indicated by solid line). Western blot analysis detected COX-2 and p42/44 MAPK phosphorylation. The results shown are the mean ± S.E. (n ≥ 3), expressed as a percentage of the maximal level of p42/44 MAPK phosphorylation, displayed graphically on the right.

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