Figure 4

Histology of livers from rats subjected to BDL. Rats were infused via the tail vein with saline (A, B), Ad5-CMV-EGFP (C, D), or Ad5-CMV-AS-TGF-β1 (E, F). The animalswere subjected to BDL (C-F) or were sham-operated (A, B) according to the time course outlined in Fig. 3A. Liver sections were examined histologically by hematoxylin (A, C, E) or Sirius red (B, D, F) staining. Note the fibrous expansion of portal areas with occasional portal to portal bridging in (D) and reduced fibrosis showing only marginal fibrous expansion in the portal areas without septa in (F). Original magnifications are ×100 (A, B) and ×200 (C-F).