Accumulation of 14
C-labeled aminopyrine in db-cAMP-stimulated gastric glands. All values are expressed as percent of a value representing the gastric acid secretion induced by db-cAMP (considered to be 100%), which was calculated separately for each of the studied subjects. Each symbol represents the data for one individual. a) Pretreatment with SNP (median = 63%; p < 0.05) or SNAP (median = 81%; p < 0.05) to release exogenous NO before adding db-cAMP to stimulate acid secretion reduced the accumulation of 14C-aminopyrine in gastric glands, as compared to levels of secretion seen in untreated glands. This indicates that NO can inhibit acid secretion in gastric glands isolated from humans. b) Treatment with L-NAME to inhibit NOS in the gastric glands increased the accumulation of 14C-aminopyrine after stimulation with db-cAMP (median = 152%; p < 0.05). Those results indicate that NO inhibits db-cAMP-induced acid secretion. The NO substrate L-arginine reduced the accumulation of 14C-aminopyrine in db-cAMP-stimulated glands (median = 77%; p < 0.05). Background accumulation (bg) is also shown.