Figure 5
From: Wnt-reporter expression pattern in the mouse intestine during homeostasis
Characterization of epithelial differentiation markers in Wnt-activated cells. (A-C) Co-incubation of antibodies to β-gal (red) and UEA-1 (green), a lectin to mark Paneth and goblet cells, identifies distinct Wnt-activated cells (arrow) and overlapping expression (arrowhead). (D-F) Similar results are observed for the Paneth-cell-specific marker, cryptidin (green) when co-stained with β-gal (red). (G-I) Co-localization is not observed with dual staining of β-gal (red) and the enteroendocrine marker serotonin (5-HT; green). Solid white line marks the epithelial-mesenchymal boundary of the intestinal crypt. Bar = 25 μm.