Table 1 Primer sequences for qRT-PCR.
From: Wnt-reporter expression pattern in the mouse intestine during homeostasis
Gene | Forward Sequence | Reverse Sequence |
|---|---|---|
lacZ | 5'-GATCTTCCTGAGGCCGATACTG-3' | 5'-GGCGGATTGACCGTAA TGG-3' |
gapdh | 5'-TGGCAAAGTGGA GATTGTTGCC-3' | 5'-AAGATGGTGATGGGCTTCCCG-3' |
wnt3 | 5'-CAAGCACAACAATGAAGCAGGC-3' | 5'-TCGGGACTCACGGTGTTTCTC-3' |
wnt6 | 5'-TGCCCGAGGCGCAAGACTG-3' | 5'-ATTGCAAACACGAAAGCTGTCTCTC-3' |
wnt9b | 5'-AAGTACAGCACCAAGTTCCTCAGC-3' | 5'-GAACAGCACAGGAGCCTGACAC-3' |
sfrp2 | 5'-AGGTCCTTTGATGCTGACTGTAAA-3' | 5'-TCGGCTTCACCTTTTTGCA-3' |
c-myc | 5'-AGCTTCGAAACTCTGGTGCATAA-3' | 5'-GGCTTTGGCATGCATTTTAATT-3' |