Figure 6

TGF-β1, collagen-1, and α-SMA mRNA levels and measurement of hepatic hydroxyproline content. After BM-MSC administration, the mRNA relative expression levels of TGF-β1 (A), collagen-1 (B), and α-SMA (C) through quantitative real-time PCR were significantly decreased 1 ± 0.27, 4.61 ± 0.61, and 2.14 ± 0.35 (P <0.01) and 1 ± 0.39, 14.04 ± 4.54, and 4.74 ± 2.46 (P <0.01) and 1 ± 0.32, 8.83 ± 3.12, and 2.62 ± 0.41 (P <0.01) in G1, G2, and G3, respectively. (D) Measurement of hepatic hydroxyproline content was quantified colorimetrically from liver tissues. Quantitative analysis showed that the contents of hepatic hydroxyproline of the liver tissue were 16.44 ± 4.07, 85.81 ± 17.62, and 46.25 ± 13.19 (P <0.01) in G1, G2, and G3, respectively. Values are expressed as means ± SD. *P <0.01.