Skip to main content
Figure 2 | BMC Gastroenterology

Figure 2

From: Congenital lactose intolerance is triggered by severe mutations on both alleles of the lactase gene

Figure 2

Intracellular localization of wild type and the mutants in COS-1 cells. For visualization of LPH, transiently transfected COS-1 cells, expressing the wild type LPH, LPH-Y1473X or LPH-D1796fs, were fixed with 4% paraformaldehyde and permeabilised with 0.5% saponin. Immunolabeling was carried out using mAb anti-LPH (1:1000) as the primary antibody and anti-mouse IgG conjugated with Alexa 488 as the secondary antibody (1:500). The samples were analyzed by confocal laser microscopy.

Back to article page