Table 1 Oligonucleotide primer pairs used for RT-qPCR analysis
From: Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn’s disease
Oligo name | GC % | Sequence |
|---|---|---|
TNFα Sense | 50 | CGAACATCCAACCTTCCAAAC |
TNFα Anti-Sense | 42 | TGGTGGTCTTGTTGCTTAAAGTTC |
iNOS Sense | 42 | CATCAACAACAATGTGGAGAAAGC |
iNOS Anti-sense | 55 | TCTGCTGCTTGCTGAGGTTG |
IL-6 Sense | 48 | GACAGCCACTCACCTCTTCAG |
IL-6 Anti-sense | 50 | GGAAGGTTCAGGTTGTTTTCTGC |
IL-8 Sense | 52 | CAGCCACTACAAACAGAGCACTG |
IL-8 Anti-sense | 48 | CAAAGGGATGACAAGCAGAAAG |
IL-23 Sense | 54 | GGA CAA CAG TCA GTT CTG CTT GC |
IL-23 Antisense | 50 | GGA GGC TGC GAA GGA TTT TG |
IL-10 Sense | 55 | CCAAGACAACACTACTAAGGCTCCTTT |
IL-10 Antisense | 48 | GCTTCTTATATGCTAGTCAGGTA |
COX-2 Sense | 59 | CTCCTATTATACTAGAGCCCTTCCTC |
COX-2 Anti-sense | 58 | TTTTCCAATCTCATTTGAATCAGG |
CD163 Sense | 48 | AGGGTGATAGAAGAGGCCAACACT |
CD163 Anti-sense | 50 | TTGCACCGGACAAACTTCATGGC |