Fig. 3
From: The role of cytoplasmic p57 in invasion of hepatocellular carcinoma
p57 regulates the expression of p-cofilin in the cytoplasm. a Confocal immunofluorescence microscopy analysis of BEL7402-shNC, BEL7402-shp57, SMMC7721-shNC and SMMC7721-shp57 cells. Green fluorescence represents the transfected plasmid, red fluorescence shows the expression of the target protein, and blue fluorescence shows the nucleus. b Western blot analysis of nuclear and cytoplasmic extracts of p-cofilin in BEL7402-shNC, BEL7402-shp57, SMMC7721-shNC and SMMC7721-shp57 cells. β-actin was used as an internal control for cytoplasmic proteins. Lamin A was used as an internal control for nuclear proteins. GFP, Green fluorescent protein; TRITC, tetramethylrhodamine; DAPI, 4′,6-diamidino-2-phenylindole