Table 2 Primers for RT-PCR analysis
Gene | Accession number | Species | Primer sequences (5′-3′) |
|---|---|---|---|
TNF | NM_000594 | Homo Sapiens | Fw: CGCTCTTCTGCCTGCTGCACT |
Rev: ACTGGAGCTGCCCCTCAGCTT | |||
IL1B | NM_000576.2 | Homo Sapiens | Fw: AAAGCTTGGTGATGTCTGGTC |
Rev : GGACATGGAGAACACCACTTG | |||
PTGS1 (COX1) | NM_000962.2 | Homo Sapiens | Fw: TCCTGTTGGTGGACTATGG |
Rev: GTGGTGGTCCATGTTCCTG | |||
PTGS2 (COX2) | NM_000963.2 | Homo Sapiens | Fw: TGGGAAGCCTTCTCTAACCTC |
Rev: TCAGGAAGCTGCTTTTTACCTT | |||
PLA2G4A (cPLA2) | NM_024420.2 | Homo Sapiens | Fw: TGCTACCTACGTTGCTGGTCT |
Rev: TTCTCTGGAAAATCAGGGTGA | |||
PTGDS (L-PGDS) | NM_000954.5 | Homo Sapiens | Fw: AGAAGAAGGCGGCGTTGTCC |
Rev: CCACCACTGACACGGAGTAGG | |||
HPGDS | NM_014485.2 | Homo Sapiens | Fw: GAGAATGGCTTATTGGTAACTCTGT |
Rev: AAAGACCAAAAGTGTGGTACTGC | |||
MRPS6 | NM_001010 | Human mouse rat | Fw: CCAAGCTTATTCAGCGTCTTGTTACTCC |
Rev: CCCTCGAGTCCTTCATTCTCTTGGC | |||
PTGDR (DP1) | NM_000953 | Homo Sapiens | Fw: CCTGGAGGAGCGGATCA |
Rev: GCTCCATAGTAAGCGCGATAAA | |||
PTGDR2 (DP2) | NM_004778 | Homo Sapiens | Fw: CCTGTGCTCCCTCTGTGC |
Rev: TCTGGAGACGGCTCACTG | |||
PPARG | NM_138711.3 | Homo Sapiens | Fw: AAAGTCGTCCTTCCCGCTGACCAAA |
Rev: GATGGCCACCTCTTTGCTCTGC |