Fig. 4

Phosphorylation of Rb protein in MiaPaCa-2 cells that were cocultured with NIH-3 T3 cells with/without FAP. a Immunoblots for phosphorylated Rb (P-Rb) using MiaPaCa-2 cell lysates that were cocultured with NIH 3 T3 cells with/without FAP. b The P-Rb expression level was normalized to that of tubulin. The experiments were repeated three times. The black and gray bars indicate MiaPaCa-2 cells after coculture with FAP-expressing NIH-3 T3 cells and MiaPaCa-2 cells after coculture with NIH-3 T3 cells without FAP expression, respectively. *, P < 0.05