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Fig. 2 | BMC Gastroenterology

Fig. 2

From: Novel exonic mutation inducing aberrant splicing in the IL10RA gene and resulting in infantile-onset inflammatory bowel disease: a case report

Fig. 2

Loss of function of the IL-10 receptor. Functional analysis of IL-10 receptor complex was performed by determining signal transducer and activator of transcription 3 (STAT3) phosphorylation using flow cytometry. Peripheral blood mononuclear cells from the patient (right panel) and his father (left panel) were stimulated for 15 minutes with IL-10 (20 ng/mL) or IL-6 (20 ng/mL) or kept unstimulated, and later fixed, permeabilized and stained for phosphorylated STAT3 (pSTAT3), which is downstream of the IL-10 and IL-6 receptor complexes

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BMC Gastroenterology

ISSN: 1471-230X

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