Fig. 3

Niclosamide induced PERK activation and the expression of PERK downstream genes in hepatoma cells. a QGY7701 and HepG2 cells were planted in 6-well plates and cultured overnight. Cells were fed with fresh complete DMEM medium (10%FBS) with indicated concentration of niclosamide or DMSO. Cells were harvested and lysed with 1 % SDS lysis buffer after 24 h of niclosamide treatment. 30 μg of total protein was seperated by SDS-PAGE for immune-blotting. Commercial primary antibodies were used to probe target protein or phosphorylated protein on membrane. Signal of western blotting was captured by Tanon Gel Image system. b Results of western blotting were analyzed with Gel Image system software (Tanon) and data were presented as ratio of target protein to GAPDH in the form of grayscale value. c Relative phosphorylated eIF2α level was analyzed with western blotting and data were presented as ratio of phosph-eIF2α to total eIF2α in the form of grayscale value