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Fig. 6 | BMC Gastroenterology

Fig. 6

From: Niclosamide induced cell apoptosis via upregulation of ATF3 and activation of PERK in Hepatocellular carcinoma cells

Fig. 6

ATF3 was involved in modulation of cell apoptosis induced by niclosamide. a ATF3 knockdown HepG2 cell line (HepG2-ATF3KD) and control cell lines (GFP knockdown, HepG2-Control) was treated with indicated chemicals for 24 h and cells were harvested and lysed with 1 % SDS lysis buffer. 30 μg of total protein was seperated by SDS-PAGE for western blotting assay. Indicated primary antibodies were used for immunoblotting. HepG2-ATF3KD and HepG2-control cells were planted in 96-well plates and maintained (b) without or with (c) 10 μM of niclosamide for 3 days. Cell number was countered every day. Data from three independent experiments were analyzed and presented as average ± SD. ** indicates p < 0.01. D and E, HepG2-ATF3KD and HepG2-Control cells were treated with or without 10 μM of niclosamide for 24 h. Then, total RNA was extracted and cDNA was synthesized. Relative mRNA level of (d) PERK and (e) CHOP were analyzed with qRT-PCR. Data was presented as average ± SD. *p < 0.05,**p < 0.01

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