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Table 1 Krebs buffers and concentrations

From: The Ussing chamber system for measuring intestinal permeability in health and disease

Buffer Concentration
Krebs Stock 136 mM NaCl, 1.5 mM CaCl2*2H2O, 4.3 mM KCl, 1.6 mM KH2PO4, 27 mM NaHCO3, 1.4 mM MgSO4*7H2
Glutamate Stock 114 mM Glutamate
Sodium Pyruvate Stock 115 mM Sodium Pyruvate
Glucose Stock 200 mM Glucose
Mannitol Stock 200 mM Mannitol
Transport Krebs 115.6 mM NaCl, 1.3 mM CaCl2*2H2O, 3.7 mM KCl, 1.4 mM KH2PO4, 23 mM NaHCO3, 1.2 mM MgSO4*7H2
Glucose Krebs 5.8 mM Sodium Pyruvate, 5.7 mM Glutamate, 10 mM Glucose
Mannitol Krebs 5.8 mM Sodium Pyruvate, 5.7 mM Glutamate, 10 mM Mannitol