Fig. 4

MiR-122 directly binds to the 3′UTR of TLR4 transcript. a Prediction of miR-122 targets in TLR4 3′UTR. The complementary sequences between miR-122 and TLR4 3′UTR (GenBank accession: NM_138554.4). b The mutated nucleotides in the putative targets of TLR4 and the sequencing data of the mutated pTLR4-MUT. The pTLR4-MUT were generated to minimize the match of miR-122 with TLR4 3′UTR in the region of nt1603-nt1609. The red boxes are the mutated regions. c MiR-122 mimic was co-transfected into 293 T cells with TLR4-WT or TLR4-MUT plasmid, respectively. TLR4 expression in the treated cells was observed at 48 h post-transfection. Values are presented as the mean ± SEM (n = 4). * refers to P < 0.05, ** refers to P < 0.01