Table 1 List of Primers used for quantitative RT-PCR assays
Gene | Forward | Reverse |
|---|---|---|
ER⍺ | 5′- CCTCCCGCCTTCTACAGGT-3′ | 5′- CACACGGCACAGTAGCGAG-3′ |
ERβ | 5′-CTGTGATGAACTACAGTGTTCCC-3’ | 5′-CACATTTGGGCTTGCAGTCTG-3’ |
nNOS⍺ | 5′-GGTGGAGATTAACATTGCTGTCCTA-3′ | 5′-TTCTCCATGTGTTTGATGAAGGACT-3′ |
Nrf2 | 5′-TCTCCTCGCTGGAAAAAGAA-3′ | 5′-TAAAGCACAGCCAGCACATT-3’ |
DHFR | 5′-TCGACCATTGAACTGCATCGTCGCC-3’ | 5′-GGAATGGAGAACCAGGTTTTCCTACC-3’ |
GCH-1 | 5′- GAGCATCACCTTGTTCCATTTG − 3′ | 5′- GCCAAGTTTACTGAGACCAAGGA − 3′ |
β-Actin | 5′-TGGAATCCTGTGGCATCCATGAAAC-3 | 5′-TAAAACGCAGCTCAGTAACAGTCCG-3′ |