Figure 1

Fluorescent microscope images of small bowel preparations from patients with IBS A. Chlamydia LPS in EEC-like cells with apical nuclei and strong basal immunofluorescence (arrows). (Monoclonal FITC-conjugated antibody with Evans blue; original magnification × 63). B. Chlamydia LPS in a few cells within the epithelium (thick arrows) and l. propria (thin arrows). (Monoclonal FITC-conjugated antibody with Evans blue; original magnification × 63). C. Chlamydia trachomatis MOMP-positive immunofluorescence within 2 EEC-like cells (arrows) and 4 cells within l. propria (arrowheads). (Mouse MOMP-antibody and FITC-conjugated rabbit anti-mouse antibody; original magnification × 63). Hoechst (DAPI conjugated) for nuclear staining. D-F. Immunostainings for (D) Chlamydia LPS (FITC, green), (E) chromogranin A (Alexia 568, red), and (F) merged showing co-localisation of chromogranin A and Chlamydia LPS in enteroendocrine cells and Chlamydia LPS in l. propria. G-I. Immunostainings for (G) Chlamydia LPS (FITC, green,) (H) CD68 (Alexia 350. blue), and (I) merged showing co-localisation of CD68 and Chlamydia LPS in macrophages (arrows). Three enteroendocrine cells are also positive for Chlamydia LPS (arrowheads).