Figure 2
Histopathological examination of mouse livers revealed fibrosis and HCC but no evidence of cirrhosis. (A) Liver tissue from all control mice at each time point gavaged with olive oil alone revealed normal cellular architecture (upper, H-E, original magnification ×40). Liver tissue from CCl4 Group at Time point I revealed some damage of liver cells and centrilobular congestion with no infiltration of inflammatory cells (bottom left, H-E, original magnification ×200). Liver tissue from CCl4 Group at Time point II had increased mitotic activity (arrows), intense neutrophilic infiltration, extensive fatty changes and severe centrilobular necrosis (bottom middle, H-E, original magnification ×600). Liver tissue from CCl4 Group at Time point III revealed well-differentiated HCCs (arrowheads) with severe fatty changes (bottom right, H-E, original magnification ×40). High-power view (×200) of tumor cells is shown. (B) Masson's trichrome staining of the liver from all control mice at each time point revealed normal lobular architecture and a normal distribution of collagen (upper). Masson's trichrome staining of liver tissue from CCl4 Group at Time point II revealed extensive collagen deposition and pseudolobular formation, suggesting liver fibrosis (bottom middle). The degree of collagen deposition decreased at Time point III (bottom right). (original magnification ×40).