Table 1 Set of primers used in Real-time quantitative PCR
From: Effect of intracellular lipid accumulation in a new model of non-alcoholic fatty liver disease
Gene name | Accession number | Forward | Reverse |
|---|---|---|---|
Interleukin L6 | NM_000600 | ACAGATTTGAGAGTAGTGAGGAAC | GGCTGGCATTTGTGGTTGG |
Interleukin 8 | NM_000584 | GACATACTCCAAACCTTTCCAC | CTTCTCCACAACCCTCTGC |
Tumor necrosis factor alpha | NM_000594 | GTGAGGAGGACGAACATC | GAGCCAGAAGAGGTTGAG |
Transforming growth factor beta | NM_000660 | GCAACAATTCCTGGCGATACC | CTCCACGGCTCAACCACTG |
Alpha 2 macroglobulin | NM_000014 | AACCAGGACAAGAGGAAGGAAG | AGATAAGCGAGGAGCACATAGG |
Vascular endothelial growth factor A | NM_003376 | TCGCTTACTCTCACCTGCTTC | TTCCAACAATGTGTCTCTTCTCTTC |
Nerve growth factor | NM_002506 | AGGAGCAAGCGGTCATCATC | TCTGTGGCGGTGGTCTTATC |
Connective tissue growth factor | NM_001901 | CGAGGAGTGGGTGTGTGAC | CAGGCAGTTGGCTCTAATCATAG |
Thrombospondin 1 | NM_003246 | ACCAACCGCATTCCAGAGTC | CCGCACAGCATCCACCAG |
Insulin-like growth factor 2 | NM_000612 | CGCTGCTACCGCCATCTC | GTCCCTCTGACTGCTCTGTG |