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Table 3 Primers designed for targeting the mucosal inflammatory cytokine-encoding genes in hamster model

From: The protective effect of recombinant Lactococcus lactis oral vaccine on a Clostridium difficile-infected animal model

Gene

Accession no.

5′ to 3′ primer sequence

Amplicon size

Gro-1

NM_008176

S: GCCACCCGCTCGCTTCTCTG

282bp

  

AS: TTACTTGGGGACACCTTTTA

 

APRO

X15267

S: GGAAGGCCGTGGTGCTGATGG

412bp

  

AS: CCGAAGGAGAAGGGGGAGATG

 

ICAM-1

NM_010493

S: GGGCACCCAGCAGAAGTTG

512bp

  

AS: CCAGCCGAGGACCATACAGC

 

IL-6

NM_031268

S: TTGGGACTGATGCTGGTGACA

205bp

  

AS: GTGCATCATCGTTGTTCATACAATC

 

MCP-1

NM_012333

S: CCCCACTCACCTGCTGCTACT

352bp

  

AS: CACTGTCACACTGGTCACTCCTAC

 
  1. S and AS denote the sense and antisense primers, respectively.
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BMC Gastroenterology

ISSN: 1471-230X

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