Table 1 c-KIT gene oligonucleotide primer
c-KIT exon sequence (5'-3') | length of PCR product (bp) | annealing temperature (°C) |
|---|---|---|
9 (F) 5'-TCC TAG AGT AAG CCA GGG CTT T-3' | 283 | 56 |
9 (R) 5'-TGG TAG ACA GAG CCT AAA CAT CC-3' | Â | Â |
11 (F) 5'-CTG AGA CAA TAA TTA TTA AAA GGT GA-3' | 227 | 60 |
11 (R) 5'-TTA TGT GTA CCC AAA AAG GTG ACA-3' | Â | Â |
13 (F) 5'-GCT TGA CAT CAG TTT GCC AG-3' | 193 | 60 |
13 (R) 5'-AAA GGC AGC TTG GAC ACG GCT TTA-3' | Â | Â |
17 (F) 5'-TAC AAG TTA AAA TGA ATT TAA ATG GT-3' | 228 | 56 |
17 (R) 5'-AAG TTG AAA CTA AAA ATC CTT TGC-3' | Â | Â |