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Fig. 4 | BMC Gastroenterology

Fig. 4

From: Expression profiling and intracellular localization studies of the novel Proline-, Histidine-, and Glycine-rich protein 1 suggest an essential role in gastro-intestinal epithelium and a potential clinical application in colorectal cancer diagnostics

Fig. 4

Immunofluorescent staining of PHGR1 protein in differentiating Caco-2 cells. Caco-2 cells grown on filters for 2 (a, d, g), 5 (b, e, h), 9 (c, f) and 14 (i) days were stained with antibodies against human PHGR1 (green). The cells were counterstained with Alexa594-conjugated antibodies against the tight-junction protein occludin (red) and the DNA dye DAPI (blue). See Additional file 3 for negative isotype control staining of the Caco-2 cells. a-c Images taken with a 10× objective. Scale bars = 100 μm. d-f Images taken with a 100× objective. Scale bars = 10 μm. g-i Images taken with a confocal laser scanning microscope at 60× magnification. Scale bars = 10 μm. Z-X and Z-Y views are shown in the side panels

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BMC Gastroenterology

ISSN: 1471-230X

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