Table 2 Antibodies
Dilution | Company | Catalogue number | |
|---|---|---|---|
Primary antibodies | |||
pGSK3β-Ser9 | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 5558 |
pβ-catenin-Ser552 | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 5651 |
pAkt-Ser473 | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 4060 |
GSK3β | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 12456 |
β-catenin | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 8480 |
Akt | 1:1000 (1% BSA/PBS) | Cell Signalling Technology, Danvers, USA | 4691 |
β-Actin | 1:2500 (0.5% LFM/PBS) | Sigma-Aldrich, Taufkirchen, Germany | A1978 |
Secondary antibodies | |||
Goat anti-rabbit | 1:7500 (0.5% LFM) | Jackson Immuno Research, Suffolk, UK | 111–035-045 |
Goat anti-mouse | 1:7500 (0.5% LFM). | Jackson Immuno Research, Suffolk, UK | 115–035-068 |