Table 1 Sequences of real-time PCR primers used in this study
Gene | Primer Sequences(5′-3′) |
|---|---|
TLR4 | F: 5′-TTGAGCAGGTCTAGGGTGATTGAAC-3′ |
R: 5′-ATGCGGACACACACACTTTCAAATA-3′ | |
GAPDH | F: 5′-ATCACTGCCACCCAGAAGAC-3′ |
R: 5′-TTTCTAGACGGCAGGTCAGG-3′ | |
miR-122 | RT: 5′-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGC ACTGGATACGACCAAACA-3′ |
F: 5′-GGGTGG AGTGTGACA ATGG-3′ | |
R: 5′-TGCGTGTCGTGGAGTC-3′ | |
U6 | RT: 5′-CGCTTCACGAATTTGCGTGTCAT-3′ |
F: 5′-GCTTCGGCAGCACATATACTAAAAT-3′ | |
R: 5′-CGCTTCACGAATTTGCGTGTCAT-3′ |