Fig. 3

Negative ion collision-induced dissociation mass spectra of a the parent ions m/z 510 and m/z 480 confirming these atypical bile acids as the glyco-sulfate and taurine conjugates, respectively, of 3β-hydroxy-5-cholenoic acids. b The LC–MS/MS mass chromatograms of the specific ion transitions for both compounds compared with a reference standard of the glyco-sulfate conjugate of 3β-hydroxy-5-cholenoic acid and of tauro-lithocholic acid