The gut microbiota composition in patients with right- and left-sided colorectal cancer and after curative colectomy, as analyzed by 16S rRNA gene amplicon sequencing

Table 3 Metabolic function prediction of the microbiota by PICRUSt2 of the RCC and control groups

Variable	P-value
Upregulation (RCC > C)
Maltose 6′-phosphate phosphatase^{) a)}	0.0043
Sulfoquinovose isomerase^a)	0.0094
D-alanine-poly(phosphoribitol) ligase^a)	0.0125
Dodecanoyl-[acyl-carrier-protein] hydrolase	0.0127
Phosphoenolpyruvate carboxykinase (GTP)^a)	0.0135
Mevalonate kinase^b)	0.0138
DNA-3-methyladenine glycosylase II	0.0141
(2Z,6E)-farnesyl diphosphate synthase^b)	0.0146
Diphosphomevalonate decarboxylase^b)	0.0147
Phosphomevalonate kinase^b)	0.0150
Downregulation (C > RCC)
2-Aminoethylphosphonate-pyruvate transaminase	0.0011
Ferredoxin hydrogenase	0.0026
Adenosylmethionine-8-amino-7-oxononanoate transaminase	0.0031
Dethiobiotin synthase	0.0036
UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase	0.0040
Kdo(2)-lipid IV(A) lauroyltransferase	0.0041
UDP-3-O-acyl-N-acetylglucosamine deacetylase	0.0042
Fumarate reductase (quinol)^c)	0.0042
3-Deoxy-8-phosphooctulonate synthase	0.0043
Succinate dehydrogenase (quinone)^c)	0.0043

Using enzyme classification (EC) numbers, we tabulated the top 10 enzyme-encoding genes with highly significant differences between the RCC and control groups. Each metabolism-related enzyme was classified according to function within the range that could be discriminated as follows. ^a) sugar metabolism-related; ^b) cholesterol-related; ^c) short-chain fatty acid-related

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