Fig. 3

RHOB and SPIDR were selected from genes enriched in the “response to oxidative stress” term for verification. (A-B), analysis of H3K27ac signals at the RHOB (A) and SPIDR (B) loci in HCC and normal liver (NL) tissues based on the GSE112221 dataset. The SE of RHOB was divided into three constituents (SE1, SE2 and SE3). The SE of SPIDR was divided into four constituents (SE1, SE2, SE3 and SE4). (C-D), ChIP-qPCR was used to detect the H3K27ac levels at the SE constituents of RHOB (C) and SPIDR (D) loci in L02, HepG2 and Hep3B cells. **P < 0.01, vs. L02. (E-H), qRT-PCR and Western blotting were employed to detect the mRNA (E-F) and protein (G-H) expression of RHOB and SPIDR in HepG2 and Hep3B cells, respectively. HepG2 and Hep3B cells were treated with DMSO or JQ1 (2, 5 and 10 µM). **P < 0.01. (I), effect of JQ1 on ROS levels of HepG2 and Hep3B cells. **P < 0.01. (J-K), ChIP-qPCR was used to detect the H3K27ac levels at the SE constituents of RHOB (J) and SPIDR (K) in HepG2 and Hep3B cells treated with DMSO or JQ1 (2, 5 and 10 µM). *P < 0.05, **P < 0.01. Data for C-F and I-K were shown as mean ± SD of three independent experiments.