Fig. 6

NRF1 bound to the SE of SPIDR to drive its transcription. (A), ChIP-qPCR was used to measure the binding between NRF1 and the four SE constituents of SPIDR. **P < 0.01 vs. IgG. (B-C), qRT-PCR was used to detect the mRNA levels of NRF1 (B) and SPIDR (C) in HepG2 and Hep3B cells transfected with siNC or siNRF1. **P < 0.01 vs. siNC group. Ns, non-significant vs. blank group. (D), Western blotting was used to detect the protein levels of NRF1 and SPIDR in HepG2 and Hep3B cells transfected with siNC or siNRF1. Data for A-C were shown as mean ± SD of three independent experiments.