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Fig. 3 | BMC Gastroenterology

Fig. 3

From: Effect and potential mechanism of oncometabolite succinate promotes distant metastasis of colorectal cancer by activating STAT3

Fig. 3

HO-3867 attenuates succinate-enhanced migration and invasion of colorectal cancer cells. (A-C) the effect of succinate on STAT3 and p-STAT3 expression levels were assessed by western blot assays after HO-3867 interfered with STAT3 phosphorylation. (E) wound healing migration assays to observe the effect on the colorectal cancer cell lines SW480 and HCT116; wound healing was photographed at 0 and 24 h. (D) Quantitative analysis of the wound-healing rate. (G) Cell migration and invasion assays were used to test the effect of succinate on the migration and invasion of colorectal cancer cells; (Magnification, 100x). (F, H) Quantitative analysis of transwell membrane cells. (I) CCK-8 cell proliferation assays to determine the effect of succinate on the activity of colorectal cancer cell lines. (It was performed under the same conditions and time as in Fig. 2, all samples derive from the same experiment and that gels/blots were processed in parallel)

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BMC Gastroenterology

ISSN: 1471-230X

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